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KMID : 1101320100420020071
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Korean Journal of Clinical Laboratory Science
2010 Volume.42 No. 2 p.71 ~ p.80
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Patterns of Antimicrobial Resistance and Genotyping of Carbapenemase-producing Imipenem-nonsusceptible Pseudomonas aeruginosa
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Lee Jin-Hee
Lee Gyu-Sang
Lim Kwan-Hun
Eom Yong-Bin
Kim Shin-Moo
Kim Jong-Bae
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Abstract
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Pseudomonas aeruginosa are important nosocomial pathogens. Their resistance to carbapenem is increasing and causing concerns in Korea. An increasing prevalence of carbapenem resistance mediated by acquired carbapenemase is being reported. Over a 10 month-period from July 2007 to April 2008, 32 strains of imipenem-nonsusceptible P. auruginosa were isolated from Kangwon National University Hospital. To determine the prevalence and genotypes of the carbapenemase-producing clinical isolates, the antibiotic susceptibility was determined by Microscan Walkaway 96 SI System and the carbapenem activity was detected by the modified Hodge test and the imipenem-EDTA-SMA double-disk synergy test. The metallo-β -lactamase gene and OXA-type β-lactamase gene reported in Korea were detected by PCR. As for the result of PCR, 30 isolates of P. aeruginosa were found to have blaIMP-1-like and 1 isolate was found to have blaIMP-1-like and blaVIM-2. No clinical isolates were found to have blaSIM-1, blaOXA-23-like and blaOXA-24-like. Random amplified polymorphic DNA (RAPD)-PCR and dendrogram for genetical similarity to band patterns of each clinical isolates were examined. P. aeruginosa were grouped into 7 clusters of up to 50% of similarity index. In the P. aeruginosa group, PS3 was resistant to the most antibiotics, PS1 was susceptible to the most antibiotics. PS7 was resistant to aztreonam unlike other groups. This is the first report of prevalence of carbapenemase in Chuncheon.
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KEYWORD
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Pseudomonas aeruginosa, Carbapenemase, blaIMP-1-like, blaVIM-2, RAPD-PCR
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